Prevalence of invA Gene of Salmonella spp. in Fish and Fishery Resources from Manila Bay Aquaculture Farms Using Real-Time PCR

Manila Bay contributes significantly to the Philippines’ economy through industry, trade, tourism, and agriculture. However, microbiological contamination in bay is putting it at risk present. Pathogen of water poses a serious threat food safety, implicating public health. To address these concerns, present study examined prevalence Salmonella six different aquaculture commodities, Perna viridis (mussel), Crassostrea iridalei (oyster), Scylla serrata (crab), Penaeus spp. (shrimp), Oreochromis niloticus (tilapia), Chanos chanos (milkfish), as well environmental from growing areas. A tandem approach culture-based real-time PCR methods for isolation identification was carried out. accurately identify isolates, Taqman assay based on invA gene used. Among fishery resources examined, no positive samples were observed C. O. niloticus. In addition, found twenty (20) samples, representing 16.26% all commodities collected. Furthermore, 13.58% 81 examined. P. had highest 55.55% out 18 Samples contaminated with failed meet regulatory limits set by BFAR FAO 210 series 2001 EC No. 2073/2005. that sample matrix significant impact presence (p < 0.05). spatial temporal distribution did not vary greatly > This underscores importance imposing strict policies bodies prevent diseases, thus avoiding severe health implications.